The department operates five regional offices . Each office supervises the fisheries, wildlife, law enforcement, and parks staff within their region. These offices are valuable contacts for constituents seeking information about resources and staff in their regions. The Office of the Secretary , in Topeka, houses the office of KDWPT Secretary Robin Jennison and various administrative and legislative staff. The Pratt Operations Office , near Pratt, houses the offices of division directors of the Fisheries and Wildlife , Administrative Services, Law Enforcement , and Parks divisions. This office oversees staff who manage game, fish, nongame , and threatened and endangered species statewide. The Pratt office is also home to the department's Information & Education Section, which produces   Kansas Wildlife & Parks magazine , brochures, news, and various educational programs. The KDWPT website is also maintained from this office. The Emporia Research & Survey Office is responsible for fish and wildlife research. The Kansas City District Office handles requests and materials for this highly populated area.

KDWPT manages 25 state parks  and the Prairie Spirit Trail. Most provide utility and primitive camping, and access to reservoirs, trails, and wildlife areas. Many parks host annual events such as concerts, festivals, and competitions.

KDWPT operates or participates in six museums/nature centers : the Great Plains Nature Center in Wichita, the Milford Nature Center near Junction City, the Prairie Center in Lenexa, and the Pratt Education Center next to the Pratt Operations Office near the city of Pratt, the Kansas Wetlands Education Center at Cheyenne Bottoms north of Great Bend and the Southeast Kansas Nature Center in Galena. These education centers display wildlife dioramas, native fish tanks, and various educational wildlife exhibits. In addition, the centers offer public programs on an ongoing basis and by request.

Kansas has four fish hatcheries , at Meade , Farlington , Milford , and Pratt . These hatcheries are responsible for producing some 44 million sportfish annually for stocking in public waters across the state. Species include channel catfish; largemouth, smallmouth, and spotted bass; striped bass and wipers; and walleye, saugeye, and sauger.

For hunters, birdwatchers, and other wildlife enthusiasts, KDWPT boasts 63 wildlife areas , ranging in size from less than one hundred acres to nearly 20,000 acres. In addition, the federal government operates eight wildlife refuges . These areas harbor game species such as deer, turkey, pheasant, waterfowl, quail, squirrel, and rabbit, as well furbearing animals, including raccoon, badger, bobcat, opossum, fox, and muskrat. Hundreds of songbird species also may be observed at different times of the year.

KDWPT offers anglers and boaters both state fishing lakes and federal reservoirs . State fishing lakes are smaller bodies of water, anywhere from two acres to nearly 350 acres, managed primarily for fishing only. Most of these waters do not allow skiing or swimming. The state's federal reservoirs, ranging in size from 1,200 acres to 16,000 acres, offer fishing, boating, and swimming. Most federal reservoirs are also home to a state park, as well as a state or federal wildlife area, or both.

Kansas also boasts more than 200 small local community lakes , many of them stocked and managed by KDWPT for fishing.

Public river access in Kansas is limited. Only the Kansas, Arkansas, and Missouri rivers are public. However, there are numerous river access points to these rivers, and other streams are available to the public in public wildlife areas and under the Fishing Impoundments and Stream Habitat (F.I.S.H.) program .