The Stippled Darter lives in brooks near sources of springs. It prefers small, clear pools or recesses beneath cut-banks where it conceals itself near large stones, in hanging roots, or fallen leaves. They also occupy shallow overflow and seepage pools, maintained by ground water, alongside channels of larger streams. This fish probably spawns in April in Kansas. Its reproductive habits and food source are unknown.
The Stippled Darter barely enters Kansas where it occurs rarely in the Spring River drainage.